Determination of Anthocyanin Chloride in Purple Sweet Potato by HPLC / 中药新药与临床药理

ABSTRACT

Objective To develop a HPLC method for the determination of anthocyanin chloride in purple sweet potato.Methods The analysis was carried out by using Kromsail C18 column(4.6 mm? 250 mm,5 ? m).The mobile phase consists of acetonitrile-water-acetic acid(15 ∶ 76.5 ∶ 8.5).The wavelength of detection was at 530 nm.Results The linear range of anthocyanin was 0～ 0.208 9 ? g(r=0.999 8).The average recovery was 97.95 %(RSD=1.0 %,n=5).Conclusions This method is simple and reliable.