Establishment of osteosarcoma pulmonary metastasis in rat model / 重庆医科大学学报

ABSTRACT

Objective:To establish the pulmonary metastasis model of osteosarcoma in rats.Methods:UMR106 cells(rat osteosarcoma strain) were cultured in RPMI1640 media and collected before transplantation and resuspended in serum-free media or collagen gel to a final concentration of 1?107 cells/ml.About 200?l cell suspensions were injected into the right proximal tibia of 3-week-old Sprague-Dawley rats.Twelve rats were used in every group sorted by resuspension fluid.All rats were treated by cyclosporin A and SMZ-TMP after transplantation.The dates of tumor appearance and rat death were noted,the size of tumor was measured once a week,the metastasis foci on the lung surface was counted and the limbs afflicted by tumor were observed by X-ray after death.Both the tumors in limb and lung were stained with hematoxylin and eosin,and analyzed by microscopy.Results:All rats developed local intratibial bone tumors that were radiographically and histologically similar to primary human osteosarcoma.The pulmonary metastasis foci were found in all rats at autopsy.The tumor formation time of serum-free media group was(10.0?1.65) days,shorter than that of collagen gel group,which was(17.8?0.87) days(P