Determination of Sinapine Thiocyanate in Semen Sinapis Albae of Different Processed Products / 中国中医药信息杂志

ABSTRACT

Objective To develop an HPLC method for the assay of sinapine thiocyanate in Semen Sinapis Albae of different processed products. Methods It was assayed by RP-HPLC. The column was Hypersil BDS Phenyl (5 ?m, 250 mm?4.6 mm), the mobile phase was acetonitrile-0.08 mol/L KH2PO4 (15∶85). The UV detection wavelength was 240 nm. The flow rate was 1.0 mL/min. Results Good linearity of sinapine thiocyanate was showed within the range of 0.8～4.0 ?g (r=0.999 7). The average recovery was 100.98% (RSD=3.51%). Conclusion The method is simple, accurate and good reproducible. It can be used for quality control of Semen Sinapis Albae of different processed products.