Establishment of a two-dimensional electrophoresis technology on colon carcinoma / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2007.
Artigo
em Chinês
| WPRIM
| ID: wpr-577515
ABSTRACT
Objective:
To establish a two-dimensional electrophoresis technology tissue protein of colon carcinnma.Methods:
Lysis buffer of different volume was taken to extract tissue proteins of colon carcinoma,and different protein quantities (1mg,2mg,3mg) different preparation ways were taken to establish a two-dimensional electrophoresis. Coomassie brilliant blue was applied to stain protein,and patterns were analyzed.Results:
With a molecular mass between 6.5~200ku and isoelectric points (pI) from 3~10,1mg proteins obtained 569 protein spots,2mg 786,and 3mg 835. Pattern of 2mg protein and the sample two were the best.Conclusion:
A two-dimensional electrophoresis technology on tissue protein of colon carcinoma has been established successfully.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Chongqing Medical University
Ano de publicação:
2007
Tipo de documento:
Artigo
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