Your browser doesn't support javascript.
loading
Establishment of a two-dimensional electrophoresis technology on colon carcinoma / 重庆医科大学学报
Journal of Chongqing Medical University ; (12)2007.
Artigo em Chinês | WPRIM | ID: wpr-577515
ABSTRACT

Objective:

To establish a two-dimensional electrophoresis technology tissue protein of colon carcinnma.

Methods:

Lysis buffer of different volume was taken to extract tissue proteins of colon carcinoma,and different protein quantities (1mg,2mg,3mg) different preparation ways were taken to establish a two-dimensional electrophoresis. Coomassie brilliant blue was applied to stain protein,and patterns were analyzed.

Results:

With a molecular mass between 6.5~200ku and isoelectric points (pI) from 3~10,1mg proteins obtained 569 protein spots,2mg 786,and 3mg 835. Pattern of 2mg protein and the sample two were the best.

Conclusion:

A two-dimensional electrophoresis technology on tissue protein of colon carcinoma has been established successfully.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Chongqing Medical University Ano de publicação: 2007 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Chongqing Medical University Ano de publicação: 2007 Tipo de documento: Artigo