Establishment of a two-dimensional electrophoresis technology on colon carcinoma / 重庆医科大学学报

ABSTRACT

Objective:To establish a two-dimensional electrophoresis technology tissue protein of colon carcinnma. Methods: Lysis buffer of different volume was taken to extract tissue proteins of colon carcinoma,and different protein quantities (1mg,2mg,3mg) different preparation ways were taken to establish a two-dimensional electrophoresis. Coomassie brilliant blue was applied to stain protein,and patterns were analyzed. Results:With a molecular mass between 6.5～200ku and isoelectric points (pI) from 3～10,1mg proteins obtained 569 protein spots,2mg 786,and 3mg 835. Pattern of 2mg protein and the sample two were the best. Conclusion:A two-dimensional electrophoresis technology on tissue protein of colon carcinoma has been established successfully.