The effect of alveolar typeⅡcell to lung fibroblast in the rat model of pingyangmycin induced pulmonary fibrosis / 重庆医科大学学报

ABSTRACT

Objective:To investigate the FSP1mRNA, TGF-?mRNA and HGFmrNA expressinn of lung fibroblasts being affected by alveolar typeⅡcells in pingyangmycin-induced pulmonary fibrosis in rats and its possible mechanism. Methods: Twenty-eight SD rats were randomly divided into two groups as the Pingyangmycin group and saline group. Pulmonary fibrosis was induced by intralracheal instillation with pingyangmycin in the Pingyangmycin group, and the saline group was treated with normal saline, Then ten rats in Pingyangmvcin group and four rats in saline group were sacrificed respectively at the 7th,28th day after intratracheal instillation. Three sacrificed rats in the Pingyangmyein group and three sacrificed rats in the saline group were abstracted alveolar typeⅡcells. The six sacrificed rats in the Pingyangmycin group were abstracted lung fihroblasts. When the two kinds of cells were at the good stale, the experiment groupput the supernate fluid of alveolar typeⅡcells of Pingyangmycin group into the lung fihroblasts of Pingyangmycin group;the control group put the supernate fluid of alveolar typeⅡcells of saline group into the lung fibroblasts of Pingyangmycin group, then coculture 48 hours, the levels of FSP1mRNA,TGF-?mRNA, HGFmRNA of lung fibroblasts were detected by demi-quantitate RT-PCR. The rest rats of Pingyangmycin grnup and the saline group were used HE stained. Results:(1)The expression of FSP1mRNA, TGF-?mRNA, HGFmRNA in experiment group were higher than the control group at the 7 th,28th day (P0.05),except the level of HGFmRNA in experiment group at the 28th was higher than at the 7th (P