Content Determination of Monotropein in the Different Parts of Morinda officinalis and Its Counterfeit Species / 中药新药与临床药理

ABSTRACT

Objective To determine the content of monotropein in the different parts of Morinda officinalis and in its counterfeit species.Methods The method of HPLC was used with chromatographic conditions as followsKromasil C18(150 mm?4.6 mm,5 ?m) column,the mobile phase of methol-0.4 %phosphate solution(595→28.871.2,15 min),the velocity of flow being 1 mL/min,the detection wavelength at 231 nm and column temperature being 25 ℃.Results The content of monotropein in the leaves and radix of Morinda officinalis and Morinda parvifolis is the highest.Conclusion It is reasonable to replace the root of Morinda officinalis with the leaves of Morinda officinalis and Morinda parvifolis for the monotropein extraction.