Supercritical CO_2 extraction of essential oil from Valeriana officinalis by orthogonal design / 中成药

ABSTRACT

AIM:To study the parameters of supercritical CO_2 extraction of essential oil from valeriana efficinalis by orthogonal design. METHODS: Four factors,such as extraction pressure,extraction temperature,extraction time and separation temperature were chosen by the observation of orthogonal design,each factor was assigned to three levels.Bornyl acetate content was selected as a marker in a position to determine optimal extraction. RESULTS: Pressure and temperature were the main factors in effecting the extraction of bornyl acetate,extraction time was minor factor relatively. CONCLUSION: The optimal extraction is as follow,parameters were extraction pressure12 MPa,extraction temperature45 ℃,extraction time1 hour,separation temperature35 ℃,it gave the best recoveries of essential oils and bornylacetate.