Determination of oridonin and rosemarinic acid in Donglingcao Tablets by HPLC with changing ultraviolet-visible wavelength / 中成药

ABSTRACT

AIM:To establish the method for determining oridonin and rosemarinic acid in Donglingcao Tablets(Rabdosia rubescens(Hemsl.) Hara). METHODS:Oridonin and rosemarinic acid were determined by HPLC simultaneously with changing ultraviolet-visible wavelength.Chromatographic condition was composed of ODS-C_(18) column,methanol-03.% phosphoric acid (40∶60),UV detection wavelength of oridonin at 238 nm and of rosemarinic acid at 329 nm. RESULTS:The average recoveries were 97.8% and 96.7% and related standard deviations(RSD) were 1.9% and 2.3%. CONCLUSION:This method can be used to determine simultaneousely oridonin and rosemarinic acid in Donglingcao Tablets.