Experimental study on treatment of human gastric cancer cell line SGC7901 with combination of TRAIL and paclitaxe / 重庆医科大学学报

ABSTRACT

Objective:To investigate the effect and potential mechanism of combined paclitaxe and tumor necrosis factor-related poptosis-inducing ligand(TRAIL) on apoptosis of human gastric cancer cell in vitro.Methods:SGC7901 cells were cultured with paclitaxe at different concentrations(1,5,10,20,40?g/ml) and different time(12,24,36,48h).Methyl thiazolyl tetrazolium (MTT) assay was used to measure the anticancer activity of paclitaxe.The ability of TRAIL alone,paclitaxe alone and TRAIL in combination with paclitaxe to induce apoptosis was measured by a flow cytometer.Expression of DR4,DR5 mRNA was examined by RT-PCR.Results:The apoptosis rate of control group(C group),paclitaxe group(P group),TRAIL group(T group) and combination group(T+P)in 24h were 2.09%,10.65%,7.79% and 24.51% respectively.T+P group was significantly higher than that in C group,T group and P group(P