Determination of Emodin and Chrysophanol in Niuhuang Qingwei Pills by HPLC / 中国中医药信息杂志

ABSTRACT

Objective To establish a quality standard of Niuhuang Qingwei Pills.Methods Hypersil C18 was used with methanol-0.1% Phosphoric acid as mobile phase.The flow rate was 1.0 mL/min,detective wavelength was 254 nm,column temperature was 30 ℃ and injection valume was 10 ?L.Results Emodin and chrysophanol showed a good linear relationship in the range of 0.04～1.60 ?g(r =0.999 9).The average recovery was 99.92%(RSD=0.6%).Conclusion The established method is simple,feasible and reproducible,and can be used for the quality control of Niuhuang Qingwei Pills.