EXPRESSION AND REGULATORY FUNCTION OF INTER RELATED POLYPEPTIDES DURING DEVELOPMENT OF RENAL TUBULES IN CHICK EMBRYO / 解剖学报

ABSTRACT

Objective To observe the expression of EGFR,TGF-?,AQP-2 and Bax during development of renal tubules in chick embryo,and explore the role and regulating significance of them. Methods The expression of EGFR,TGF-?,AQP-2 and Bax were observed by immunohistochemistry combined with stereological methods in 36 chicks kidney at 10th-46th incubation stages.The expression intensity of them was analyzed by IPP software. Results The epithelial cells of proximal tubules in chick embryo appeared EGFR immunoreactivity at 26th-46th stages;At 26th-37th stages the epithelial cells of proximal tubules and distal tubules of mesonephros appeared TGF-? immunoreactivity,the epithelial cells of proximal tubules and distal tubules of metanephros appeared TGF-? immunoreactivity at 33rd-40th stages,at 40th-46th stages the epithelial cells of proximal tubules of metanephros appeared TGF-? immunoreactivity but the epithelial cells of distal tubules did not appeare TGF-? immunoreactivity;The epithelial cells of collecting tubules in chick embryo appeared AQP-2 immunoreactivity at 26th-46th stages;The epithelial cells of proximal tubules and distal tubules of mesonephros appeared Bax immunoreactivity at 26th-37th stages,the epithelial cells of proximal tubules of metanephros appeared Bax immunoreactivity at 33rd-46th stages.Images showed the expressions of EGFR,TGF-?(?_1,?_2,?_3)and Bax first displayed up then down,but expression of AQP-2 always displayed up during development of chick embryo.Conclusion Mesonephros of chick embryo has the function of excreting.Degeneration of renal tubules of mesonephros might be related with the excessive expression of TGF-? and Bax.Development,differentiation and maturation of renal tubules and collecting tubules of metanephros might be due to the synergetic expression of EGFR,TGF-?,AQP-2 and Bax.AQP-2 relats to the balance of water during development and maturation of renal tubules in chick embryo.