Antitumor fraction from liquid submerged fermentation of Ganoderma sinense / 中草药

ABSTRACT

Objective To study the antitumor fraction from liquid submerged fermentation of Ganoderma sinense. Methods The antitumor active fractions were extracted from the hyphostroma which was produced by liquid submerged fermentation and their antitumor activities were observed by mice transplant tumor model. The total polysaccharides were tested by phenol-sulphuric acid method and the contents of reducing sugars were measured by DNS method. The cytotoxic activities were tested by MTT method in vitro. Results The optimum fraction was the intracellular soluble precipitation (sample B) by water extracting with alcohol sedimentation. The contents of total polysaccharide and reducing sugar in the optimum active fraction from liquid submerged fermentation of G. sinense were 88.4% and 3.15%, respectively, but no protein. The inhibitory ratios were 63.94% and 58.32% on the growth of H22 and Lewis tumor transplanted in mice by ig administration in the concentration of 40.5 mg/kg, respectively. The IC50 on four tumor cells of A549, LoVo, CEM, and QGY-7703 were 160, 29.28, 45.06, and 37.38 ?g/mL, respectively, which suggested that the active fraction had weak cytotoxic on LoVo, CEM, and QGY-7703. Conclusion The active fraction from liquid submerged fermentation of G. sinense is polysaccharide which has better inhibitory rate on the growth of H22 and Lewis tumor transplanted in mice but their cytotoxicities are weak.