Haemodialysis patients sera induced angiogenesis in vitro by oxidative stress / 重庆医科大学学报

ABSTRACT

Objective:To prove that haemodialysis patients sera medium can induce angiogenesis by oxidative stress. Method: Eleven haemodialysis patients were administered 400mg/d of vitamin E for 6 weeks per os (treated group),and another eleven haemodialysis patients were treated regularly(non-treated group).HUVEC were cultured in 10% treated patients sera medium and 10% non-treated patients sera medium respectively. The plasma level of MDA was detected by TBA method. Angiogenesis were observed under inverted microscope,cell proliferation examinded by MTT,cell migration measured in transwell,and ROS investigated by colorimetry. Results:In treated group,the plasma level of MDA was lower after treatment.There were tube formation(average area as 1 438?m2?112?m2) and more cell proliferation and migration after cells were cultured in non- treated haemodialysis patients sera.The production of ROS was higher than that of cells exposed to treated patients sera medium.Conclusion:Haemodialysis patients sera medium can induce angiogenesis and oxidative stress is the important mechanism.