Changes of endogenous hormone and gene expression during process of somatic embryogenesis in Panax ginseng / 中草药

ABSTRACT

Objective To study the relationship during exogenous hormone-endogenous hormone-idiogene, and establish the foundation to illuminate the molecule mechanism during process of somatic embryogenesis in Panax ginseng. Methods AFLP Analysis on the period of different culture in development pathway of P. ginseng was carried out simultaneously, the endogenous IAA, ABA, and the saponin content in the period of different cultures during process of somatic embryogenesis in P. ginseng were determined. Results It indicated the content of IAA was the highest in early embryo period and ABA was the highest in the mature embryo period. The ratio of ABA/IAA was higher at the stage of mature embryo than that of others. The gene expression was different in the different period cultures during process of somatic embryogenesis in P. ginseng. The total saponin content of test-tube plantlet is four fold higher than that in the period of cotylen embryo. Conclusion The condition that maintains normal development of somatic embryogenesis in P. ginseng is the presence of IAA. ABA correlates with the embryo priming, somatic embryogenesis and development. Especially, it influences the later period of somatic embryogenesis. It produces the different types of gene regulation in different physiology condition or different culture conditions. There is idiogene expression in the different development processes, which induces the different cell differentiation. With gradually completion of morphogenesis, it is the accumulative process of secondary metabolite.