Determination of three nucleosides in Radix isatidis by HPLC / 中成药

ABSTRACT

AIM:To establish a method for determining guanosine, uridine,and andadenosine for Radix Isatidis. METHODS: The Prevail C_(18) analytical column(4.6 mm?250 mm,5 ?m)was used.The mobile phases consisted of water and acetonitrile with gradient.The flow rate was 0.5 mL/min and column temperature was at 25 ℃.The detection wavelength was at 254 nm. RESULTS: The method had good linear relationship within the range(1.92)-38.4 ?g/mL;1.75-35 ?g/mL; 2.12-42.4 ?g/mL of guanosine,uridine and andadenosine,respectively,with the correlation coefficients of 0.999 9.The average recoveries of the three nucleosides were 99.64%(RSD=0.38%),98.94%(RSD=0.32%) and 98.85%(RSD=0.48%),correspondingly.CONCLUSION: The method is fast,rapid,convenient,accurate and can be used for quality control of Radix isatidis.