Pharmacokinetics Study of Danshensu in Danqi Injection in Dogs / 中药新药与临床药理

ABSTRACT

Objective To study the pharmacokinetics of Danshensu (tanshinol) in Danqi Injection(DQI) in dogs. Methods HPLC-UV detection was used to determine the Danshensu level in biological samples. The Lichrospher C18 column(4.6?250 mm,5?m) was used as an analytical column with a mobile phase consisted of acetonitrile-1 %acetic acid (8∶92),the flow rate being 1.0mL?min-1 and the wave-length being 280 nm. Results After intravenous injection of DQI in dogs,the plasma concentration-time curve of DQI in dogs fitted well to a two-compartment model,with the characteristics of fast absorption and slow elimination.Its pharmacokinetics parameters are as follows:?=1.533 h-1,?=0.5111 h-1,T(peak)=0.25 h,T1/2?=0.573 h. Conclusion The pharmacokinetics of DQI in dogs fits the two-compartment model.In the pharmacokinetics of Danshensu,elimination course is the main course with the slow and lasting characteristics.