Experimental Study on Buyang Huanwutang in Inhibiting Astroglial Reactivity in Vitro / 中国中医药信息杂志

ABSTRACT

Objective To study the effect of Buyang Huanwutang in inhibiting the astroglial reactivity induced by injury in vitro. Methods After cultured the spinal astroglia from newborn Wistar rats for fifteen days, cells were divided into two groups, Buyang Huanwutang group and control group without Chinese herb. Nicked the bottom of cultivated cells and a injured strip of astroglia cells of 1 mm width 15 mm length was made. Immunocytochemistry method was used to demonstrate the expression of glial fibrillary acidic protein (GFAP). Results From 2 to 48 hours after the nick, GFAP expression and the astroglial reactivity were apparently inhibited by Buyang Huanwutang. Conclusion Buyang Huanwutang can inhibit the astroglial reactive proliferation induced by injury in vitro.