Nonlinear Regression Analysis of Chief Chemical Components in the Combinations of Different-dose Herbs of Wutou Decoction / 中药新药与临床药理

ABSTRACT

Objective To observe the changes of chief chemical components(hypaconitine,ephedrine,pseudoephedrine,peoniflorin,glycyrrhetic acid) in the combinations of different-dose herbs of Wutou Decoction(WD). Methods Uniform design method was adopted to design the experiments,and HPLC was applied to determine the contents of chief chemical components of hypaconitine,ephedrine,pseudoephedrine,peoniflorin,glycyrrhetic acid. DPS V7.55 was used to carry out the nonlinear regression analysis of experimental data such as decocting rates of the chief chemical components. The mathematical model was established to analyze the experimental results. Finally,with the decocting rates of the chief chemical components as the optimizing indexes,the combination of optimal-dose herbs of WD was seeked. Results There existed an interaction between each herb of WD,and each herbs as well as the interaction between the herbs had a certain effects on the decocting rate of the chemical components. In particular,the interaction of prepared Radix Aconiti and prepared Radix Glycyrrhizae had obvious effects on the decocting rates of hypaconitine and glycyrrhetic acid. On the basis of the different optimization index,different combinations of optimal-dose herbs of WD can be obtained. Conclusion Through the method of nonlinear regression analysis,rich connotation of compatibility in Chinese herbal formula can be obtained.The optimization of optimal-dose herbs combination of the formula should be based on the pharmacological actions.