Effects of hehodge signaling pathway on poliferation,apoptosis and cyclinD1 expression of breast cancer cell line MDA-MB-231 / 重庆医科大学学报

ABSTRACT

Objective:To research the effects of HH singaling pathway on proliferation,apoptosis and CyclinD1 expression of breast cancer MDA-MB-231 cells by MDA-MB-231 cells treated with Cyclopamine,a HH singaling pathway inhibitor.Methods:MDA-MB-231 cells were treated with Cyclopamine.Cell proliferation was detected by MTT;Cell cycle distribution and apoptosis were analysed by flow cytometry(FCM).The mRNA levels of GLI1,CyclinD1 were measured by reverse transcription-polymerase chain reaction(RT-PCR);The protein level of CyclinD1 was detected by immunostaining of cell lines.Results:Compared with control group,Cyclopamine could significantly inhibit the proliferation of MDA-MB-231 cells in a dose-and time-dependent manner;By FCM the treatment of MDA-MB-231 cells with Cyclopamine induced a remarkable increase in the proportion of cells in the G0/G1 phase of the cell cycle and after 48 h the sub-G1 peak appeared.The mRNA levels of GLI1,CyclinD1 and the expression of CyclinD1 protein were reduced.Conclusion:The HH signaling pathway was activeted in MDA-MB-231 cells.HH signaling pathway inhibitor,Cyclopamine,could inhibit the proliferation,induce the apoptosis and reduce the expression of CyclinD1 in MDA-MB-231 cells.