Early effects of vitreous cryopreservation with tetramethylpyrazine on immunological response of nerve allograft / 重庆医科大学学报

ABSTRACT

Objective:To observe the change of immunity in vivo after Vitreous cryopreservation with tetramethylpyrazine nerve allograft was implanted into the recipient so as to judge its immunogenicity.Methods:Sciatic nerves of Sprague-Dawley rats were resected and made by Vitreous cryopreservation with tetramethylpyrazin.The nerves from Sprague-Dawley rats were implanted into the dorsal and ventral subcutis of Wistar rats in experimental group。Fresh nerves from Sprague-Dawley rats were implanted into the dorsal and ventral subcutis of Sprague-Dawley rats in control group respectively.T lymphoeytes infiltraion in isograft nerve was investigated by immunohis-tochemical Methods:staining CD4 and CD8 positive T lymphocytes with anti-CD4 and CD8 monoclonal antibodies(MoAb).Results:320 mg/L tetramethylpyrazine combined vitreous cryopreservation stainings at-20℃for 3 weeks showed that the number of CD4 and CD8 positive T lymphocytes infiltrated into the nerve implants of the experimental group was much lower than that of other tetramethylpyrazine combined vitreous cryopreservation in the control group.there was no marked difference of the 320mg/L tetramethylpyrazine combined vitreous cryopreservation stainings at-20℃for 3 weeks nerves group compared with autografting group.Conclusion:The nerve treated by 320 mg/L tetramethylpyrazine combined vitreous cryopreservation at-20℃for 3 weeks shoued lower immunogenicity after implanta-tion,and there was no significant rejection response after transplantation in rats.