Preparation of doxorubicin liposome and their effect on human MCF-7 / 重庆医科大学学报

ABSTRACT

Objective:To prepare doxorubicin liposome and investigate its effect on human MCF-7 in vitro. Methods:DOX liposome was prepared by(NH4)2SO4-gradient method.The MTT method was used to test cytotoxicity and reverse multidrug resistance of DOX liposome on MCF-7/DOX. Results:DOX was encapsulated by 90.77%. The results of liposome MTT experiment suggested that IC50 of free DOX was 4 times bigger than DOX liposome.The DOX liposomes’reversing effect on MDR was 4 times as much as that of free DOX. Conclusion:The method of(NH4)2SO4 gradient is suitable for producing DOX liposome and it has better cytotoxicity and reversing MDR effect on MCF-7 than free DOX.