Expression and significance of estrogen receptor ? and ? in endometrium of rats with polycystic ovary syndrome / 重庆医科大学学报

ABSTRACT

Objective:To determine the expression of estrogen receptor ? and estrogen receptor ? (ER?、ER?) in the endometrium of rats with polycystic ovary syndrome(PCOS). Methods: PCOS model was made by dehydroepiandrosterone(DHEA)and insuline(n=30). The serum hormone levels were examined by radioimmunoassay. The expression of the mRNA and protein of ER? and ER? in endometrium of twogroups were observed by using reverse transcription polymerase chain reaction(RT-PCR)and immunohistochemistry. Results: The expression of protein of ER? in PCOS group in endometrium was significantly higher than those in the control group(P0.05). RT-PCR analysis showed the same expression of ER? mRNA and ER? mRNA in endometrium as immunohistochemistry. Conclusion: ER? in endometrium of PCOS rats is expressed excessively.