Determination of main antiviral alkaloidal constituent in Radix Isatidis and its preparation by HPLC-DAD / 中成药

ABSTRACT

AIM: To determine epigoitrin and 2,4(1H,3H)-quinazolinedione in Radix Isatidis and its preparation by HPLC-DAD. METHODS: HPLC was carried out with an Agilent 1200,equipped with ZORBAX SB-C_(18) column(4.6 mm?250 mm,5 ?m).The mobile phase of acetonitrile(0.1% phosphoric acid+0.03% triethylamine)aqueous solution=12∶88.The flow rate was 0.7 mL/min.The detection wavelength was set at 224 nm.The column temperature was at 30 ℃. RESULTS: The linear ranges of epigoitrin was 0.040 9 ?g-1.103 8 ?g;2,4(1H,3H)-quinazolinedione was 0.010 6 ?g-0.169 6 ?g,The recoveries of epigoitrin and 2,4(1H,3H)-(quinazolinedione) were 98.44%(n=9),with RSD of 1.43% and 98.67%,with RSD of 1.65%,respectively. CONCLUSION: The method is simple,sensitive and reliable.It can be used for quantitative determination of Radix Isatidis and its preparation.