Effects of Kangliyin on N Gene Relative Expression in the Infectious Bronchitis Virus Infected Model / 中药新药与临床药理

ABSTRACT

Objective To observe the therapeutic effect of Kangliyin(KLY,the compound Chinese herbal formulation with the functions of clearing heat and damp elimination,regulating stomach and dissipate phlegm) on the models with infection of infectious bronchitis virus(IBV),and to evaluate its effect on the IBV N gene relative expression by using real-time florescent quantitative polymerase chain reaction(PCR).Methods Seven-day-old chicks were inoculated with IBV by the nasal and ocular administration.The chicks were divided into 6 groups by stratified random sampling according to the body weight.The 6 groups were blank control group,model group,three KLY treatment groups(receiving KLY at the dosage of 10 g/kg? d-1,20 g/kg? d-1 or 40 g/kg? d-1,respectively),and positive control group(ganciclovir at the dosage of 0.004 g/kg? d-1).The chicks in the model group and blank control group received normal saline,and the medication groups received the corresponding medicine according to the experimental design from the next day of inoculation,and the treatment lasted 5 days.The relative quantity of IBV N gene expression in the lung and bronchial tissues were assayed by real-time florescent quantitative PCR,and the clinical manifestations and the pathological changes of all groups were also examined.Results Compared with the model group,KLY could significantly inhibit the IBV N gene expression,improve the general clinical symptoms and respiratory symptoms,and improve the pathological changes in the bronchus and lung of the IBV infected model.Conclusion KLY could effectively inhibit the viral replication,repair the pathological damage in the bronchus and lung,and improve the general clinical symptoms in the IBV infected model,which is beneficial to the recovery of the chicks from IBV infection.