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Relationship between Wnt/?-Catenin signal pathway in gastric carcinoma cell line SGC-7901 and the expression of Caspase-8,Caspase-3 and Bcl-2 / 重庆医科大学学报
Journal of Chongqing Medical University ; (12)2003.
Artigo em Chinês | WPRIM | ID: wpr-579770
ABSTRACT

Objective:

To study the regulatory mechanism of Wnt/?-Catenin passthway-influenced apoptosis of human gastric cancer line SGC-7901 by detecting the expression of ?-Catenin mRNA,Bcl-2mRNA,Caspase-8mRNA and Caspase-3mRNA.

Methods:

(1) Expressions of ?-Catenin,Bcl-2,Caspase-8 and Caspase-3mRNAs in groups with or without indomethacin induction were detected by RT-PCR.(2) Methyl thiazolyl tetrazolium(MTT) assay was used to measure the anticancer activity of indomethacin.

Results:

(1)As compared with groups without indomethacin induction,expression of ?-Catenin mRNA and Bcl-2 mRNA were lower,however,increased expression of Caspase-8 and Caspase-3mRNA were found in SGC-7901 cells after treatment with indomethacin.(2) Indomethacin of 50 ?mol/L at 24 h couldn't induce apoptosis of gastric cancer cells.Indomethacin of 50?mol/L at 48 and 72 h and of 100?mol/L and 200?mol/L at 24、48 and 72 h could induce apoptosis of gastric cancer cells in a dose-and time-dependent manner.

Conclusion:

Gastric cancer cells may be caused by apoptosis inbalance through Wnt/?-Catenin passthway,which activates expression of proto-oncogene Bcl-2 and inhibites expressions of Caspase-8 and Caspase-3.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Chongqing Medical University Ano de publicação: 2003 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Idioma: Chinês Revista: Journal of Chongqing Medical University Ano de publicação: 2003 Tipo de documento: Artigo