The influence of BMP_9 in myocardiocytes differentiation of stem cell in vitro / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-579795
ABSTRACT
Objective:
ToexploreinfluenceofBMP9inmyocardiocytesdifferentiationof stem cell Invitro.Methods:
TheC3H10T1/2stem cell were transfected with 2.67 ml/L pAdEasy-BMP9 Ad-GFP plasmid for 1 week and 2 weeks .The specific transcription factors of cardiomyocytes were detected in stem cells during differentiation with plasmid transfection by RT-PCR and the specific proteins in cardiomy-ocytes were detected by laser confocal microsopy after 1 week;the change of cells ultramicro structure were detected by electron micro-scope after 2 weeks.Results:
Cells volume are increscent obviously,cells trend become unanimous,the connection between cells are compact,refractivity of cells enhance conspicuously after induction for 1 week.The expression of NKx 2.5,GATA-4,MEF2C could be de-tected in C3H10T1/2 stem cells with pAdEasy-BMP9 Ad-GFP plasmid tranfection.Cx43 and cTnT can be detected with plasmid tranfec-tion.Cardiomyocytes ultramicro structure can be detected after induction for 2 weeks.Conclusion:
BMP9 makes a very important influ-ence in C3H10T1/2 stem cells targeted differentiation into cardiomyocytes。
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Chongqing Medical University
Ano de publicação:
2003
Tipo de documento:
Artigo
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