Extraction Techniques and Quality Standards of Compound Tianqi Yizhi Granule / 中国中医药信息杂志

ABSTRACT

Objective To investigate the best conditions of extraction techniques for compound Tianqi Yizhi granule and to establish its quality standards. Methods The best conditions of extraction techniques for compound Tianqi Yizhi granule were optimized by orthogonal experiments, with the yield of total saponins as the index. The qualitative analysis of Panax Notoginseng and Ginseng with Thin-Layer Chromatography and determination of total saponins with UV-spectrophotometry was made. Results The optimal condition of total saponins was that the microwave extraction for 3 times and extracting for 30 minutes, the material-water rate of 1∶10. In TLC procedure, the control material and the test sample showed the same colored spots, while the negative control sample showed no interference. Ginsenoside Re showed a good linear relationship at the range of 40～200 ?g, Y =250.83X -0.017, r =0.997 2. The accuracy and precision were accord to the standards. Conclusion The extraction techniques and the quality standards are feasible.