Determination of Fructus Cnidii in Dafeng Wan by HPLC / 中国中医药信息杂志

ABSTRACT

Objective To establish the method for determining the content of Fructus Cnidii in Dafeng wan by HPLC. Methods Diamonsil ODS1 C18 column was used with CH3OH-0.1 mol/L (NH4)2SO4 (70︰ 30) [adjust pH to 2.5 by sulphuric acid solution (1→5)] as the mobile phase. The detection wavelength was set at 322 nm and flow rate was 1.0 mL/min. Results The calibration curve was linear at the range of 0.101～0.808 ?g for the Fructus Cnidii and linear equation was Y =9.964 406?10-3X -1.355 684?10-1, r=0.999 5. The average recovery was 98.4% and RSD was 1.40% (n=6). Conclusion This method was simple and accurate, with good specificity and repeatability, which provides scientific quantitative analysis method of Fructus Cnidii in Dafeng wan.