Effect of Rumo Shengji San on Skin Defect After Repair with Tissue-engineered Skin / 中药新药与临床药理

ABSTRACT

Objective To investigate the effect of Rumo Shengji San on promoting tissue regeneration of skin defect after repair with tissue-engineered skin.Methods The dermal scaffolds were reconstructed by collagen sponge in which human skin fibroblasts were transplanted inside and human keratocytes were transplanted on the surface.The tissue-engineered skin was transplanted into the wound surface of defected skin in naked mice.Rumo Shengji San was applied externally once every other day after transplantation for 2 days.The animals were randomly divided into two groupsexternal application group and control group,4 mice in each group.The differentiation tissue of the tissue-engineered skin was sampled 9 days after transplantation.The epidermis thickness and dermal capillary proliferation in wound of defected skin were observed with HE staining,and double-labeled immunofluorescence method was used to test dermal laminin(Ln) and typeⅠcollagen protein expression.Results Rumo Sheng Jji San promoted the development of epidermis after transplantation for 9 days.The number of capillaries in dermis was increased,and the expression of Ln and typeⅠcollagen protein was promoted in external application group than that in the control group.Conclusion Rumo Shengji San can promote tissue regeneration of skin defect after repair with tissue-engineered skin.