Influence of dendritic cells modified with ICOS extracellular region on survival of renal allografts in rats / 重庆医科大学学报

ABSTRACT

Objective:To study the influence of donor dendritic cells (DCs) modified with ICOS extracellular region on the survival of renal allografts in rats. Methods:Bone marrow derived DCs of Brown Norway(BN) rats were modified with ICOS extracellular region gene recombined adenovirus and injected into Lewis rats 24 h before BN→Lewis kidney transplantation. Survival time of renal allografts was observed and one-way mixed splenic cell reaction (MSR) of the recipients to donors and the third party rats were performed by means of MTT on the 20th postoperative day. Results:Survival time of renal allografts was prolonged significantly [comparedwith control, (23.2?3.08) d vs(8.5?1.4) d,P