Inhibitory Effect of Human Serum Containing Niaoduqing Capsule on Proliferation and Transdifferentiation of Human Renal Tubular Epithelial Cells / 广州中医药大学学报

ABSTRACT

Objective To observe the effect of human serum containing Niaoduqing Capsule(NC)on proliferation and transdifferentiation of human renal tubular epithelial cells(HK-2).Methods The in-vitro cultured HK-2 were divided into 6 groups according to the culture media normal control group,uremic serum groups(at the concentration of 5% and 10%), NC-containing serum groups(at the concentration of 5% and 10%),and Monopril group(at the concentration of 10-6mmol/L).Cell proliferation was measured by methylene blue assay.Cell life cycle and the percentage of cells with ?-smooth muscle actin(?-SMA)expression positive in every group were evaluated by flow cytometry.Results The optical absorption value in uremic serum groups was higher than that in the normal control group(P