Determination of Borneol in Aqueous Humor and Perfusate of Rabbit Cornea by Gas Chromatography / 中药新药与临床药理

ABSTRACT

Objective To establish gas chromatography for determining the contents of borneol in aqueous humor and perfusate of rabbit cornea.Methods Naphthalene was used as the internal standard,samples were extracted by ethyl acetate and the chromatographic procedure was carried out in OV-1701 capillary column.The parameters were set as followsInject Temp,210 ℃;Oven Temp,30 ℃;FID Temp,250 ℃;Carrier gasNitrogen,3.0psi;Hydrogen,45 ml/min;Air,450 ml/min;split ratio,101.Results Borneol and isoborneol were well separated from other components.Borneol and isoborneol had an good linearity in the rang of 0.2～20 ?g/ml,R2 was 0.9953 for borneol and 0.9902 for isoborneol;and the intra-day and inter-day RSD were less than 9.68 %and 12.60 %respectively.Conclusion The method is simple and precise,and can be used for determining borneol in ophthalmological preparation.