Determination of Chlorogenic Acid in Xiao'er Yinniu Granula by HPLC / 中药新药与临床药理
Traditional Chinese Drug Research & Clinical Pharmacology
;
(6)2000.
Artigo
em Chinês
| WPRIM
| ID: wpr-580338
ABSTRACT
Objective To develop an HPLC method for the determination of chlorogenic acid in Xiao'er Yinniu Granula.Methods A Phenomenex ODS C18 column (4.6 mm?250 mm,5 ?m) was used,column temperature was at 20 ℃,methanol-0.4 %phosphoric acid water solution(2080)was used as the mobile phase,the flow rate was 1 mL?min-1,and the detection wavelength was 327 nm.Results The calibration curve of chlorogenic acid was linear between 0.284~1.704 ?g (r=0.999 6),the average recovery and the relative standard deviation were 96.19 %and 1.16 %(n=5),respectively.Conclusion The method is simple,accurate and reproducible,by whihc chlorogenic acid can be better separated from Xiao'er Yinniu Granula.This method can be used for quality control of Xiao'er Yinniu Granula.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Traditional Chinese Drug Research & Clinical Pharmacology
Ano de publicação:
2000
Tipo de documento:
Artigo
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