Dynamic observation on Th1/Th2 type immune response in mice immunized with recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus / 重庆医科大学学报

ABSTRACT

Objective:To dynamically observe changes of Th1/Th2 type immune responses in mice immunized with recombinant Bifidobacteria bifidum(Bb)-Eg95-EgA31 vaccine of Echinococcus granulosus(Eg).Methods:BALB/c mice were vaccinated by 5?108 colony forming unit(CFU) orally and 5?105CFU intranasally respectively.Mice were killed on week 2,4,6,8,10,12,14,16,18 and 20 after immunization respectively,sera were collected to measure the levels of IgG,its subclasses and IgE by enzyme linked immunosorbent assay(ELISA);Spleens were removed to determine the levels of IFN-?,IL-12,TNF-?and IL-10 of splenocyte culture supernatant by ELISA.Results:Oral immunization mice showed higher levels of IgG,IgG2a,IgG2b,IgG1,IgG3 and IgE on the 8～10th week,2～20th week,2～20th week,4～8th week,6～12th week and 10th week respectively,while IFN-?,IL-12,TNF-?and IL-10 on the 2～16th week,2～12th week,2～6th week and 4～12th week post vaccination respectively;Intranasal immunization mice showed higher levels of IgG,IgG2a,IgG2b,IgG1,IgG3 and IgE during the 4～10th week,4～20th week,2～20th week,2～12th week,4～12th week and 10～12th week respectively,while IFN-?,IL-12,TNF-?and IL-10 on the 2～8th week,2～12th week,2～8th week and 6～16th week post vaccination respectively.Conclusion:Mixed Th1/Th2 type immune responses may be induced in mice immunized with the recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus.Oral and intranasal inoculation are two better immune routes,and the former is better than the latter.