The research of early diagnosis for the rat hepatom model / 重庆医科大学学报

ABSTRACT

Objective:to research how to implement early diagnosis for the hepatom model when the rats are alive.Methods:40 young male Wistar rats(30～40 d)were randomly divided into two groups,20 rats in each group(the control group and the interruptedly low doses experimental group).The control group was fed with physiologic saline for 20 weeks.The interruptedly low doses experimental group was fed with 0.2%diethylnitrosamine(DENA)at a dose of 10 mg/kg every week 5 times.The experimental group was induced for 15 continuous weeks.All of them were sacrificed at the 20th week when the rats were inspected by MRI and weighed.The histological changes of liver tissue were observed under microscope.The levels of liver function and?-foetoprotein(AFP)were recorded。all the factors were put into a logistic regression model for multivariate analysis.Results:the induction achievement ratio of hepatoma model in Wistar rats was 85%.The correct rate of MRI for hepatoma was 94.1%.With multivariate analysis,only 1 of the 7 variables in the final logistic regression model was statistically significantalanine aminotransferase(ALT).Conclusion:Through giving rats low doses of DENA interruptedly and performing MRI for inspecting pathological changes of rat livers with anomaly increased levels of plasma ALT,we can implement early diagnosis for the hepatom model to decrease the rate of death and extrahepatic metastasis when fund and time is limited.