Quality standard for Ruxinan Capsule / 中成药

ABSTRACT

AIM: To determine quantitatively active ingredients,icariine and osthole,and to identify Fructus Cnidium and Scorpio in Ruxinan Capsule ( Folium Epimedii,Fructus Cnidii,Scorpio). METHODS: Fructus Cnidium and Scorpio were identified by TLC and the contents of icariine and osthole were determined by HPLC. RESULTS: Fructus Cnidium and Scorpio were clearly separated in the TLC chromatogram. The results of HPLC analysis for icariine were a linear range of 61. 7 ng-740. 4 ng,r = 0. 999 6,an average recovery of 99. 86% with a RSD of 0. 82% and the corresponding data for osthole were a linear range of 57. 4 ng-688. 8 ng,r = 0. 999 8, an average recovery of 99. 55% with a RSD of 0. 71% . CONCLUSION: This method is simple and feasible and can be used as quality standards for Ruxinan Capsule.