The study on gene silence of STAT6 gene by RNA interference / 重庆医科大学学报

ABSTRACT

Objective:To construct the recombinant plasmid expressing STAT6 shRNA and observe the effects of RNAi suppressed STAT6 gene expression. Methods:shRNA and pIRES2-EGFP-STAT6 plasmid were cotransfected into COS7 cell,to investigate the suppression effect of shRNA on STAT6 under fluorescent microscope . This effect was confirmed by Western blot at protein expression level. Results: STAT6 protein exhibitited high expression in COS7 cells after transfecting pIRES2-EGFP-STAT6 plasmid into COS7 cells alone ; compare with the negative group,the level of the STAT6 protein in the two shRNAs group were 23.1% and 9.6%. Conclusion:STAT6 shRNA were successfully constructed.This technique deserves further study as a potential therapentic tool for asthma .