Scavenging activity of Xiaoaiping Injection for H_2O_2 and O_2～?- radicals by HPLC coupled with chemiluminescence / 中成药

ABSTRACT

AIM: To study the scavenging activities of Xiaoaiping Injection (Marsdenia tenacissima (Roxb) wight et Arn) for H2O2 and O2?- radicals by HPLC coupled with chemiluminescence and to trace the active constituents on line. METHODS: Solution of Xiaoaipin Injection was passed through HPLC to enter into the mixing tees (contained the luminol hydrogen peroxide solution and luminol pyrogallol solution,respectively),and recorded chemiluminescence signals were recorded,and their chemical structures were identified in combination with LCMS/MS. RESULTS: Three phenolic acids were identified,3-Caffeoylquinic acid,5-Caffeoylquinic acid,4-Caffeoylquinic acid,having scavenging activities for free radicals. CONCLUSION: Phenolic acids in Xiaoaiping Injection make a significant contribution to its scavenging activities for H2O2 and O2?-. It is shown that the method for on-line detection is an advantageous tool to rapidly determine antioxidants from traditional Chinese medicine.