Optimization of supercritical fluid extraction of paeoniflorin by response surface methodology / 中成药

ABSTRACT

AIM: To develop a method for optimizing of supercritical fluid extraction (SFE) of paeoniflorin. METHODS: On the basis of one-factor test and the Box-Behnken central-composite design response surface with 3 factors and 3 levels was adopted. A second order quadratic equation for SFE of paeoniflorin was built. Response surface and contour were graphed with the extraction rate of paeoniflorin as the response value. Based on the analysis of the response surface plots and their corresponding contour plots,the effects of extraction pressure,extraction temperature,and methanol concentration were explored. RESULTS: By using the new method discussed above, the optimum extraction condition was obtained as follows 18. 9 MPa extraction pressure,extraction cosolvent temperature 59. 9 ℃,68. 5% methanol was chosen as cosolvent with the flow rate of 0. 4 mL/min,and extraction time 60 min. CONCLUSION: Under the optimized condition,extraction rate of paeoniflorin on theoretical value is 7. 85 mg/g.