Reserpine treatment activates AMP activated protein kinase (AMPK)
Natural Product Sciences
;
: 157-161, 2017.
Artigo
em Inglês
| WPRIM
| ID: wpr-58162
ABSTRACT
Reserpine is a well-known medicine for the treatment of hypertension, however the role of reserpine in cell signaling is not fully understood. Here, we report that reserpine treatment induces the phosphorylation of AMP activated protein kinase (AMPK) at threonine 172 (T172) in PC12 cells. Phosphorylation of AMPK T172 is regulated by upstream signaling molecules, and the increase of phospho-T172 indicates that AMPK is activated. When we examined the FOXO3a dependent transcription by using the FHRE-Luc reporter assay, reserpine treatment repressed the FHRE-Luc reporter activity in a dose dependent manner. Finally, we showed that reserpine treatment induced the phosphorylation of AMPK as well as cell death in MCF-7 cells. These results suggest that AMPK is a potential cellular target of reserpine.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosforilação
/
Reserpina
/
Treonina
/
Células PC12
/
Morte Celular
/
Proteínas Quinases Ativadas por AMP
/
Células MCF-7
/
Hipertensão
Limite:
Animais
Idioma:
Inglês
Revista:
Natural Product Sciences
Ano de publicação:
2017
Tipo de documento:
Artigo
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