Cloning of Transcriptional Regulatory Sequences in Human ?-Fetoprotein Gene / 中国肿瘤生物治疗杂志

ABSTRACT

The 5'-flanking region of the human ?-fetoprotein(AFP) gene contains transcriptional regulatory sequences(TRSs) which up-regulate AFP gene expression with cell-specific enhancer activity in hepatoma cells. A couple of primers(Primerl 5'-TGCAAGCTTATGATTCCCAAATATC-3'; Primer 2 5'-GTCGAATTCGTGGCCTGGA TAAAGCTGAGT-3') were designed for synthesis and purification according to the known sequences of 5'-flanking region. AFPTRSs of 416 base pairs were amplified from human chromosome DNA by PCR, The identification of the AFPTRSs was confirmed to be consistent with reported sequences. The AFPTRSs can be applied to regulating the specific expression of cytokines in hepatoma cells.