Establishment of Two Cell Lines with Double Markers and Comparison between Their Abilities for Detecting Replication-Competent Retro viruses / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6)1995.
Artigo
em Chinês
| WPRIM
| ID: wpr-581861
ABSTRACT
In this study, we use retroviral vectors, LXSN (containing internal promoter) and GCEN(containing internal ribosomal entry site,IRES), which cany ?galactosidare gene (LacZ) and neomycin phosphotransferase gene (Neo_R) as double markers, to establish two cell lines, LG-3T3 and GG-3T3. The sensitivities of the two cell lines for detecting replication-competent retroviruses(RCR) in marker rescue test were compared. Our results indicated that both cell lines can be used in marker rescue test, the sentivity of GG-3T3 was 100 times higher than that of LG-3T3.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Cancer Biotherapy
Ano de publicação:
1995
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS