Construction of cDNA Library of Ovarian Carcinoma / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6)1996.
Artigo
em Chinês
| WPRIM
| ID: wpr-581892
ABSTRACT
We isolated polyA~+ mRNA direcdy from tumor tissue of ovarian carcinoma using Oligotex~(TM) Direct mRNA Kit (QIAGEN) to synthesize the first and second strand cDNA. The ds-cDNA termini were blunted with pfu DNA poly-rnerase. The blunted cDNAs were added EcoR I adaptor, and then were digested by Xho I . Small cDNA molecules(
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Cancer Biotherapy
Ano de publicação:
1996
Tipo de documento:
Artigo
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