TC-1 Stromal Cells Enhance Retro viral-Mediated Gene Transfer into Murine Marrow Hematopoietic Cells / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6)1996.
Artigo
em Chinês
| WPRIM
| ID: wpr-581918
ABSTRACT
Objective:
To investigate the optimal gene transfer protocols of hematopoietic cells mediated by retrovirus.Methods:
Murine bone marrow cells were infected by co-culture with murine bone marrow stromal cell line TC-1 or retro-virus packaging cells or retrovirus supernatant. Human mdr-1 and enhanced green fluorescent protein (EGFP) were used as report genes.Results:
Stromal cells could greatly increase the gene transfer efficiency when compared with that of supernatant transfection. Transduction efficiency was highest when infected BM cells were co-cultured with virus producer cells. Conculsion It may be clinically feasible in gene therapy to perform retroviral transduction by co-culture of target cells with stromal cells or cell lines.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Guia de Prática Clínica
Idioma:
Chinês
Revista:
Chinese Journal of Cancer Biotherapy
Ano de publicação:
1996
Tipo de documento:
Artigo
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