Induction of Antibody Response by Intramuscular Immunization with HER2/ neu Oncogene Extracellular Domain DNA in Mice / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6)1994.
Artigo
em Chinês
| WPRIM
| ID: wpr-581934
ABSTRACT
Objective:
To induce an immune response by intramuscular immunization with HER2/neu oncogene extracellular do- main (ECD) DNA and observe the effect in vivo by an abortion model in mice.Methods:
Human and rat HEH2/neu ECD gene were cloned and inserted into expressive vector pCDNA3. The DNA immunization, expression of HER2/neu ECD, antibody de- tection and abortion in immunized mice were studied.Results:
HER2/neu ECD protein expressed on muscle cells in the injected site, specific antibody to HER2/neu was induced by DNA immunization. The average of the offspring per delivery from immu- nized mice was significantly lower than that from control group, and the abortive embryos were found in uterus from immunized mice.Conclusion:
The results show that both human and rat HER2/neu ECD gene can induced effective immune response by DNA immunization.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Cancer Biotherapy
Ano de publicação:
1994
Tipo de documento:
Artigo
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