Flow injection immunoassay for insulin using a new synergistic enhanced chemiluminescence / 中华检验医学杂志
Chinese Journal of Laboratory Medicine
;
(12)2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-582135
ABSTRACT
Objective To establish a new non radioimmunoassay for detecting insulin. Methods We put together the techniques of chemiluminescent analysis, flow injection analysis and immunoassay and established a method,flow injection chemiluminescent immunoassay, for insulin determination using sandwhich procedure with the horseradish peroxdase (HRP) as label of antibody and using the detection system of synergistic enhanced chemiluminescence of para phenylpenol and sodium tetraphenylborate with HRP catalysed oxidation of luminol. Results Satisfactory results were obtained in the dynamic range 0.15~60.00 ?U with a relative coefficient of 0 997 2 ( P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Journal of Laboratory Medicine
Ano de publicação:
2001
Tipo de documento:
Artigo
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