Cloning of human preferentially expressed antigen of melanoma gene and its expression in E.coli / 医学研究生学报
Journal of Medical Postgraduates
; (12)2003.
Article
em Zh
| WPRIM
| ID: wpr-584694
Biblioteca responsável:
WPRO
ABSTRACT
Objective: To clone and express the preferentially expressed antigen of melanoma (PRAME) gene in E.coli. Methods: The cDNA encoding human PRAME gene was extracted from human AML cell line HL-60 and amplified by RT-PCR, then the PRAME gene was inserted into plasmid PGEM-T-easy. After sequenced, it was cloned into the prokaryotic expression vector pGEX-4T-2 to construct a clone with high level expression and the recombinant plasmid was cloned in E.coli. Results:The protein product reached the highest level at 5 h after IPTG induction. Conclusion: Since PRAME is only expressed at low levels in a few normal tissues and encodes an antigen recognized by autologous cytotoxic T lymphocytes, while expressed at high levels in patients with leukemia, it might be a new targeting candidate for tumor immunotherapy.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Journal of Medical Postgraduates
Ano de publicação:
2003
Tipo de documento:
Article