A study on new cell-cycle inhibitors from the metabolites of marine-derived Streptomyces flavorectus / 中国海洋药物

ABSTRACT

Aim To explore the antitumor active metabolites of marine-derived Streptomyces flavorectus Z4-007. Methods The separation procedure was guided by a flow cytometric bioassay using tsFT210 cells to examine cell cycle inhibitory activity, and various column chromatography using silica gel, Sephadex LH-20, and ODS were employed for the isolation and purification of bioactive compounds. Chemical structures were investigated by spectroscopic methods and biological activities were evaluated by flow cytometry using mouse cancer tsFT210 cells. Results Four bioactive compounds were isolated from the fermentation broth of Streptomyces flavorectus Z4-007, one of them had been identified as 1-(2,4-Dihydroxy-3,5-dimethyl-phenyl)-hexa- (E,E)-2,4-dien-1-one (1) and the other three compounds were considered to be peptide, Compound Ⅰ inhibited the cell-cycle of tsFT210 cells at the G_0/G_1 phase at higher concentrations, while at the lower concentrations compound Ⅰ inhibited the cell-cycle mainly at the G_2/M phase, accompanied with induction of apoptosis in the tsFT210 cells.Conclusion Compound Ⅰ was isolated from the metabolites of the genus Streptomyces for the first time and provided as a new cell-cycle inhibitor.