Effect of different administrations of insulin-like growth factor 1 on cerebral hypoxia-ischemia injury in neonatal rats / 临床神经病学杂志

ABSTRACT

Objective To investigate the effect of different administrations of insulin-like growth factor 1(IGF-1) on cerebral hypoxia-ischemia injury in neonatal rats.Methods Forty neonatal rats were divided into four groups control group, nasal-treated group, intravenous-treated group and sham-operated group. The two treat groups were given IGF-12.5?g (dissolved in 0.1 ml NS) intravenously or by nasal immediately after hypoxia, respectively. Normal saline (0.1 ml) was used intravenously in control group and only disassociation of common carotid artery was performed in sham-operated group. The animals were killed 24 hours after hypoxia. The expression of caspase-3 protein was determined by immunohistochemistry method, also pathological change was studied under light microscope.Results Compared to the control group, the expressions of caspase-3 protein in treat groups were obviously decreased (all (P