Screening of Gram Negative Strain Producing Alkali Cellulase and Characterization of the Enzymatic Reactions / 微生物学通报

ABSTRACT

The Gram negative strain H8005 was isolated from alkali silt on the site of paper mill. Under optimum conditions, the alkali cellulase activitig (CMCase) of shaking flask culture of H8005 was 4.2 IU/mL. The optimal conditions for the enzymatic reaction were about 55℃ and pH 8.0. At pH 7.0～9.0, the enzyme showed high activity and good stability. Mn~(2+ ), Fe~(3+) were the activators and Cu~(2+) , Pb~(2+ )were the inhibitors of the enzyme. The novel cellulase produced by H8005 showed great potential in the textile biofinishing and detergent industry.